Human Follicle Stimulating Hormone - hFSH



Data Sheet
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Human Follicle Stimulating Hormone - hFSH

Description: Human FSH is a glycoprotein produced from urine of post-menopausal women and having a total molecular mass of 35,500 Dalton. HuFSH is a heterodimeric hormone consisting of 165 amino acids chain (non-active) and 115 amino acids chain (active). HuFSH induces maturation of Graafian follicles of the ovary, promotes development of germinal cells in males and activates cytosolic tyrosine kinase. The HuFSH is purified by proprietary chromatographic techniques.
Source: Urine of post-menopausal women
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The FSH was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility: It is recommended to reconstitute the lyophilized FSH in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized FSH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution FSH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Biological Activity: ProSpec's HuFSH is biologically active compared to standard (IRP reference preparation 68/140). The activity was found to be 9780IU/mg.
Contaminents: Less than: 0.1% hCG, 0.5%TSH, 0.5% LH, 0.5%GH and 0.5%Prl. Free of HbsAg, Hormones to HIV and HCV.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of FSH as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.