Human Growth Hormone Releasing Hormone - hGHRH



Data Sheet
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Human Growth Hormone Releasing Hormone - hGHRH

Description: Synthetic Human GHRH is a single, non-glycosylated, polypeptide chain containing 29 amino acids and having a molecular mass of 3358 Dalton. Corresponds to the amino-terminal segment of the naturally occurring human growth hormone-releasing hormone consisting of 44 amino acid residues.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation : The protein (1mg/ml) was lyophilized after extensive dialyses against 1.7mg sodium phosphate buffer (0.1 mg sodium phosphate monobasic and 1.6mg sodium phosphate dibasic).
Solubility : It is recommended to reconstitute the lyophilized GHRH in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized GHRH although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution GHRH should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid composition: The free base of sermorelin has the empirical formula C 149 H 246 N 44 O 42 S. Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2 .
Amino-Acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Asp-Ala-Ile.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's HuGHRH is fully biologically active, increases plasma growth hormone concentrations by directly stimulating the anterior pituitary gland to release natural human growth hormone.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of HuGHRH.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nmAnalysis by RP-HPLC, using a calibrated solution of GHRH as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes