Polyclonal Rabbit Anti SARS Nucleocapsid Protein - PRASARSSP



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Polyclonal Rabbit Anti SARS Nucleocapsid Protein - PRASARSSP

Source: Rabbit
Background: The spike (S) glycoprotein of coronaviruses mediates viral entry into host cells.Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. It is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted HR-N and HR-C, that form coiled-coil structures within the ectodomain of the protein. Previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes.
Description: Full-length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139KDa band is detected.
Antigen: The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 1124-1140 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).
Isotype: Rabbit IgG
Formulation: 0.5µg/µl PBS containing 0.2% gelatin and 0.05% sodium azide.
Storage: Store at 4C, stable for 6 months. For long-term storage, store at -20C
Applications Western blot SARS detection.
Usage: This material for research, laboratory or further evaluation purposes.
Latest Publications: Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody. Chan PK, To WK, Ng TK, Houde M, J Med Virol 2004 Dec;74(4):517-20 Antibody responses against SARS-coronavirus and its nucleocaspid in SARS patients. Shi Y, Wan Z, Li P, J Clin Virol 2004 Sep;31(1):66-8 [Determination and comparison of anti-SARS antibody in children and adults] Liu JH, Ma SX, Wang HB, Tang SQ, Zhongguo Shi Yan Xue Ye Xue Za Zhi 2004 Apr;12(2):217-9 [The quantitative detection of anti-coronavirus antibody titer in medical personnel closely contacted with severe acute respiratory syndrome patients] Liu YN, Fan BX, Yu BX, Zhonghua Jie He He Hu Xi Za Zhi 2003 Oct;26(10):583-5 [Detection of the anti-SARS-coronavirus specific antibody levels in 156 SARS patients] Wang HB, Liu JH, Yu Y, Tian YP, Zhongguo Shi Yan Xue Ye Xue Za Zhi 2003 Oct;11(5):441-3 [Primary investigation on the changing mode of plasma specific IgG antibody in SARS patients and their physicians and nurses] Xu G, Lu H, Li Y, Zhao ZD, Beijing Da Xue Xue Bao 2003 May 31;35 Suppl:23-5