Mouse Anti Human Insulin - mAHuInsulin



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Mouse Anti Human Insulin - mAHuInsulin

Source: mouse
Physical Apperance: Sterile Filtered White lyophilized (freeze-dried) powder.
Clone: C7C9, D4B8, 7F8, 3A6, 8E2, 7F5 These clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunised with purified human insulin.
Isotype: IgG1 kappa for all clones
Specificity: Human Insulin
Reactivity: Clone C7C9 reacts with c-terminal pentapeptide of insulin b-chain. Clones 7F8, D4B8, 3A6 and 8E2 react with different antigenic determinants. Kass for clone 7F8 is 7.5 x 109M-1 Kass for clone D4B8 is 1.5 x 108M-1 Kass for clone 3A6 is 8.0 x 108M-1 Kass for clone 8E2 is 6.0 x 108M-1 Kass for clone C7C9 is 1.0 x 109M-1 Kass for clone 7F5 is 1.0 x 109M-1 These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Formulation: Each vial contains antibodies in PBS, pH 7,4, containing 0,1% of sodium azide as preservative.
Storage: Store at 4C, stable for 6 months. For long-term storage, store at -20C
Stability: Two years lyophilized, one month in solution at 4oC.
Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
Applications: Detection of insulin in two-site enzyme immunoassay. The following pairs are recommended: 7F8 - D4B8, 3A6 - 8E2 Clone D4B8 can be used in immunohistochemistry on frozen sections.
Usage: This material is offered for research, laboratory or further evaluation purposes.
Latest Publications: Decreased cortisol response to insulin induced hypoglycaemia in asthmatics treated with inhaled fluticasone propionate. Mahachoklertwattana P, Sudkronrayudh K, Choubtum L, Arch Dis Child 2004 Nov;89(11):1055-8 ERK1/2 (p44/42 MAP kinases) phosphorylate synapsin I and regulate insulin secretion in the MIN6 beta cell line and islets of Langerhans. Longuet C, Broca C, Hani EH, Endocrinology 2004 Oct 21; Long-lived Growth Hormone Receptor Knockout Mice: Interaction of Reduced IGF-1/insulin Signaling and Caloric Restriction. Al-Regaiey KA, Masternak MM, Sun L, Endocrinology 2004 Oct 21; Circulating insulin-like growth factors in patients infected with Helicobacter pylori. Baricevic I, Nedic O, Bojic B, Clin Biochem 2004 Nov;37(11):997-1001 Effects of timely insulin treatment on protection of beta cells in a rat model of type 2 diabetes mellitus. Zhou YS, Gao Y, Li B, Chin Med J (Engl) 2004 Oct;117(10):1523-9 [Influence of alcohol on insulin sensitivity and insulin receptor substrate-1 mRNA expression in rat skeletal muscle.] Zhang XZ, Ying CJ, Zhang XP, Zhonghua Yu Fang Yi Xue Za Zhi 2004 Sep;38(5):335-8