Recombinant Human Stromal Cell-Derived Factor-1 alpha (CXCL12) - rHuSDF-1a



Data Sheet
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Recombinant Human Stromal Cell-Derived Factor-1 alpha (CXCL12) - rHuSDF-1a

Description: Recombinant Human SDF-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8008 Dalton. The rHuSDF-1 is purified by proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Solubility: It is recommended to reconstitute the lyophilized rHuSDF-1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuSDF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuSDF-1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's SDF-1 is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuSDF-1 .
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a standard solution of SDF-1 as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.