Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor - rHuGM-CSF



Data Sheet
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Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor - rHuGM-CSF

Description Recombinant Human GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. rHuGM-CSF is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4±0.1.
Solubility: It is recommended to reconstitute the lyophilized rHuGM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuGM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuGM-CSF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.
Dimers and aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuGM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1 ng/ml, corresponding to a Specific Activity of 11.1x106 IU/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuGM-CSF.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Usage This material is for research, laboratory or further manufacturing purposes.