Recombinant Human Insulin - rHuInsulin



Data Sheet
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Recombinant Human Insulin - rHuInsulin

Description: Recombinant Human Insulin produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. rHuInsulin is purified by proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility: It is recommended to reconstitute the lyophilized rHuInsulin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuInsulin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuInsulin should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuInsulin is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuInsulin.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm. Analysis by RP-HPLC, using a calibrated solution of Insulin as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.