Recombinant Human Pegylated Interferon-alpha 2b - rHuPEG-IFN-a 2b



Data Sheet
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Recombinant Human Pegylated Interferon-alpha 2b - rHuPEG-IFN-a 2b

Description: Recombinant Human IFN-α 2b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. The Interferon-a 2b gene was obtained from human leukocytes. rHuPEG- IFN-α 2b is manufactured by attaching a 12,000 dalton methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of IFN-α 2b having a total molecular mass of 31,269 dlaton.. The rHuIFN-α 2b is purified by proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a (1mg/ml) solution in containing 5.55mg Sodium phosphate dibasic, 5.55mg sodium phosphate monobasic buffer, 296mg sucrose and 0.37mg tween 80.
Solubility: It is recommended to reconstitute the lyophilized rHuPEG-IFN-α 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuPEG-IFN-α 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuPEG-IFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been completely removed enzymatically.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel.
Biological Activity: ProSpec's rHuPEG-IFN-α 2b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 0.7 x 108 IU / mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuPEG-IFN-α 2b.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a calibrated solution of IFN-α 2b as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.