Recombinant Human Tumor Necrosis Factor-beta - rHuTNF-b



Data Sheet
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Recombinant Human Tumor Necrosis Factor-beta - rHuTNF-b

Description Recombinant Human TNF- Lymphotoxin) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 171 amino acids and having a molecular mass of 18645 Dalton. The rHuTNF-ß is purified by standard chromatographic techniques.
Source Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized protein with no additives.
Solubility: It is recommended to reconstitute the lyophilized rHuTNF-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuTNF-beta although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuTNF-beta should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Arg-Ser-Ser.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuTNF- is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D isless then0.05ng/ml, corresponding to a Specific Activity of 2 x107 IU/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuTNF-ß.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a calibrated solution of TNF-ß as a Reference Standard.
Usage This material is for research, laboratory or further manufacturing purposes.