Recombinant Human beta- Nerve Growth Factor - rHubeta-NGF



Data Sheet
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Recombinant Human beta- Nerve Growth Factor - rHubeta-NGF

Description: Recombinant Humanß -NGF produced in CHO is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular mass of 16,950 Dalton. The rHuß -NGF is purified by proprietary chromatographic techniques.
Source: CHO cells.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a 0.2µm filtered solution in 30mM sodium acetate pH=5.5 containing 1% HSA.
Solubility: It is recommended to reconstitute the lyophilized rHuß -NGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rHuß -NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rHuß -NGF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-His-Pro.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rHuß -NGF is fully biologically active when compared to standards. The ED50, calculated by PC12 cell line is 1.3-2 ng/ml and by DRG cell line is 0.8-1.5 ng/ml.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuß -NGF.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 1.1945 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a calibrated solution of -NGF as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.