Recombinant Murine Granulocyte Macrophage-Colony Stimulating Factor - rMGM-CSF



Data Sheet
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Recombinant Murine Granulocyte Macrophage-Colony Stimulating Factor - rMGM-CSF

Description Recombinant murine GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton. rmGM-CSF is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: It is recommended to reconstitute the lyophilized rmGM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Solubility: Lyophilized rmGM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rmGM-CSF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Stability: Lyophilized rmGM-CSF, although stable at room temperature, should be stored desiccated below 0 °C. Reconstituted rmGM-CSF is best stored refrigerated at 4 °C.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rmGM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line isless then0.2 ng/ml, corresponding to a Specific Activity of 5x106 IU/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rmGM-CSF.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a calibrated solution of mGM-CSF as a Reference Standard.
Usage This material is for research, laboratory or further manufacturing purposes.