Recombinant Murine Tumor Necrosis Factor-alpha - rMTNF-a



Data Sheet
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Recombinant Murine Tumor Necrosis Factor-alpha - rMTNF-a

Description Recombinant murine TNF-α produced in S.Lividans is a single, glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32 Dalton. The rmTNF-alpha is purified by standard chromatographic techniques.
Source Streptomyces Lividans.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Tris-HCL, pH=8, 5mM MgCl2 and 10% glycerol. Solubility: It is recommended to reconstitute the lyophilized rOLeptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rmTNF-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rmTNF-alpha should be stored at 4 °C between 2-7 days and for future use below -18 °C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D isless then0.05ng/ml, corresponding to a Specific Activity of 9x10 IU/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rmTNF-α.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. Analysis by RP-HPLC, using a calibrated solution of TNF- as a Reference Standard.
Usage This material is for research, laboratory or further manufacturing purposes.