Recombinant Rabbit Prolactin receptor-ECD - rRPrlr-ECD



Data Sheet
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Recombinant Rabbit Prolactin receptor-ECD - rRPrlr-ECD

Description Recombinant rbRPRLR-ECD produced in E.Coli is a non-glycosylated, Polypeptide chain containing 207 amino acids and having a molecular mass of 23972 Dalton. The rbRPRLR-ECD is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility: It is recommended to reconstitute the lyophilized rbRPRLR-ECD in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized rbRPRLR-ECD although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution rbRPRLR-ECD should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Lys-Pro-Phe-Ile.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's rbRPRLr-ECD is fully biologically active: its activity is determined by the dose-dependant inhibition of PRL-stimuled proliferation of Nb2 cells and by high affinity binding of oPLR and other lactogenic hormones. Refs: Bignon et al. (1994) JBC 269; 3318-24Gertler et al. (1996) JBC 271; 24482-91.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rbRPRLr-ECD.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 2.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). Analysis by RP-HPLC, using a standard solution of rbRPRLr-ECD as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.