Recombinant Murine Interleukin-1 beta - rmIL-1b



Data Sheet
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Recombinant Murine Interleukin-1 beta - rmIL-1b

Description: Recombinant Murine IL-1 b produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17500 Dalton. The rHuIL-1b is purified by proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation & packaging: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives
Solubility: The lyophilized rmIL-1b is very soluble in water and most aqueous buffers below and above the isoelectric point.
Stability: Lyophilized rmIL-1b although stable at room temperature, should be stored desiccated below 0°C. Reconstituted rmIL-1b is best stored refrigerated at 4°C.
Purity: Greater than 99.0% as determined by: Analysis by RP-HPLC.Anion-exchange FPLC.Analysis by reducing and non-reducing SDS-PAGE Silver Stained. (Limit of acceptance:≥98.0%. No more than 2% total impurities; no single impurity greater than 1%)
Amino Acid Composition: In total agreement with the expected amino acid composition of native murine IL-1b.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: murine IL-1b is fully biologically active when compared to standard. The specific activity as determined in the test of augmentation of lymphocyte prolifiration assay using mouse thymus was found to be 6 x 108 / mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of murine IL-1b.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of IL-1b as a Reference Standard.
Usage: This material is for research, laboratory or further manufacturing purposes.