Recombinant Rat Insulin Like Growth Factor-I - rrIGF-I



Data Sheet
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Recombinant Rat Insulin Like Growth Factor-I - rrIGF-I

Description Recombinant Rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton. rrIGF-I is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized after extensive dialysis against 100mM acetic acid.
Solubility It is recommended to reconstitute the lyophilized rrIGF-I in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized rrIGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rrIGF-I should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by: Analysis by RP-HPLC. Anion-exchange FPLC.Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec’s rrIGF-I is fully biologically active when compared to standards. The ED50, was calculated 2 methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to beless then30 ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be less then 10 ng/ml.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rrIGF-I.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of rat IGF-I as a Reference Standard.
Usage This material is offered for research, laboratory or further manufacturing purposes.
Refrences Shimatzu A. and Rotwein P. (1987) J. Biol. Chem. 262, 7894-7900 Upton Z. et al. (1996) J. Endocrinol. 149, 379-387