Human Chorionic Gonadotropin - hCG



Data Sheet
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Human Chorionic Gonadotropin - hCG

Description: hCG is produced from a sterile preparation of placental glucoprotein urine of post-menopausal women having a total molecular mass of 36,700 Dalton. hCG consists of 237 amino acids, chain-92 amino acids and chain-145 amino acids. The hCG is purified by proprietary chromatographic techniques.
Source: Urine of post-menopausal women
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The hCG was lyophilized from a concentrated (1mg/ml) solution with no additives.
Solubility: It is recommended to reconstitute the lyophilized hCG in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution hCG should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Biological Activity: ProSpec's hCG is biologically active compared to standard (IRP reference preparation 75/551). The activity was found to be 3559IU/mg.
Contaminents: Free of: HbsAg, Hepatitis B surface antigen and Hormones to HIV, Hepatitis C and HCV.
Protein content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm.Analysis by RP-HPLC, using a calibrated solution of hCG as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.